Effect of transduced mesenchymal stem cells with IL-10 gene on control of allergic asthma
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Congxia Hou [3] ; Fang Sun [3] ; Yalin Liang [4] ; Entezar Mehrabi Nasab [1] ; Seyyed Shamsadin Athari [2]
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[1] Tehran University of Medical Sciences
Tehran University of Medical Sciences
Irán
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[2] Zanjan University of Medical Sciences
Zanjan University of Medical Sciences
Irán
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[3] Department of Respiratory and Critical Care Medicine, Henan Provincial Chest Hospital, Zhengzhou 450008, Henan, China.
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[4] Kidney transplantation nephropathy diagnosis and treatment center, The 7th People’s Hospital of Zhengzhou, Zhengzhou City, 450016, Henan, China.
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- Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 51, Nº. 2, 2023, págs. 45-51
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Asthma is an important pulmonary disease associated with T helper lymphocyte (Th)2 dominant immune response, which can initiate allergic and inflammatory reactions. Interleukin (IL)-10 is the main immune suppressor cytokine, and mesenchymal stem cells (MSCs) have an immune-modulatory potential that can be transduced with the expression of the IL-10 gene to control pathophysiology of allergic asthma. Bone marrow’s MSCs were isolated and transduced with the expression vector that contains the expressible IL-10 gene. Then, allergic asthma mouse model was produced and treated with manipulated MSCs. Methacholine challenge test; measurement of IL-4, IL-5, IL-8, IL-13, IL-25, and IL-33; and total and ovalbumin (OVA)-specific immunoglobulin (Ig)E levels were done. Hyperplasia of the goblet cell, secretion of mucus, and peribronchiolar and perivascular eosinophilic inflammation were evaluated in lung pathological sections. IL-25, IL-33, and total IgE levels; AHR; eosinophilic inflammation; hyperplasia of the goblet cell; and secretion of mucus could be controlled in M, MV, and MV-10 groups, and the control in the MV-10 group was strong compared to M and MV groups. MSCs have immune-modulatory capacity that can control allergic asthma pathophysiology, and this effect can be strengthened and reinforced by the expression of IL-10 gene.
