Resumen de Evaluación in vitro de la capacidad de Bacillus cereus var. Toyoi para modificar la invasividad de Escherichia coli K88 y Salmonella Typhimurium en celulas IPEC-j2
G. González Ortiz, Marta Cerdà Cuéllar, M. Castillo, D. Solá Oriol, Susana María Martín Orúe
The potential of the probiotic Bacillus cereus var. Toyoi, to modify the intestinal invasiveness of Escherichia coli K88 and Salmonella Typhimurium was tested on an in vitro model with IPEC-J2 cells. For that purpose a total of 32 weaning piglets allocated into 16 pens were fed two experimental diets added or not with Bacillus cereus var.Toyoi (109 UFC/ g). After 34 days consuming the diets, animals were euthanized and ileal content pooled by pen and sterile supernatants obtained by centrifugation and filtration. Co-incubation of different strains of E. coli and S. Thyphimurium with the sterile ileal supernatants showed a significant reduction in the invasiveness of E. coli to IPEC-J2 cells (P= 0.01) for the animals that received the probiotic, without significant differences between strains. The two strains of Salmonella showed differences in their invasiveness to cells (P< 0.0001) but no differences related to experimental treatments. These results suggest the ability of Bacillus cereus var. Toyoi to interfere with quorum sensing mechanisms of E. coli in the ileum of pigs, probably repressing the expression of some virulence factors involved in the adhesion and invasion of epithelial cells.
