Resumen de Identificación de una asociación significativa entre el polimorfismo del gen de la adiponectina (ADIDOQ) porcina y los niveles séricos de LDL

Raquel Quintanilla Aguado, C. Melo, Ana Castelló Ponce, A. Zidi, D. Gallardo, A. Cánovas, J. Jordana De Pozas, I. Díaz, Ramona Natacha Pena Subirá, M. Amills

• By performing a genome scan for porcine serum lipid traits in a Duroc commercial population, we were able identify a significant QTL for low-density lipoprotein (LDL) concentration at 190 days on chromosome 13 (SSC13). The adiponectin (ADIPOQ) locus constitutes a potential candidate gene for this QTL, because the levels of this hormone are strongly correlated with LDL and triglyceride levels. Sequencing of 2.37 Kb of the pig ADIPOQ cDNA resulted in the identification of a c.*1512G>T 3’UTR polymorphism. This mutation was genotyped in our Duroc resource population by using a pyrosequencing-based approach. Association analyses with distinct statistical models evidenced that part of the variation of LDL levels is explained by the ADIPOQ genotype. This finding matches very well with results obtained in human and model organisms showing that ADIPOQ is a major determinant of the catabolism of very-low density lipoproteins, the precursors of LDL, and that variability in the 3’UTR of the human ADIPOQ gene is strongly associated with adiponectin levels and body weight.