Resumen de Efecto del tipo de yema de huevo, eliminación del plasma seminal y edad del donante en la crioconservación de semen ovino de las razas xisqueta, aranesa y caprino de la raza blanca de rasquera
W. García, Abigail Tabarez Rojas, Marta Terré Trulla, A. Ferrando, J. Jordana De Pozas, M.J. Palomo
The objective was to assess the effect of type of egg yolk (EY), presence or not of seminal plasma (sperm washing) and donor age on sperm viability post-thawing. Semen was collected from 8 rams and 6 bucks for two consecutive years, at the age of 1 and 2 years, respectively. Ejaculates of each species was mixed and divided into two aliquots, one was diluted in a Tris-based medium and centrifuged twice at 600xg for 10 minutes, while the other aliquot was not washed. Washed and unwashed ram as washed goat sperm was diluted in a freezing media supplemented with a final concentration of 15% of EY (fresh, powdered or clarified) and 5% of glycerol, meanwhile unwashed goat sperm was diluted in the same media but with 2% of EY. The results showed that in ram sperm, factors such as donor age (p<0.001), sperm washing (p<0.001) and the interaction of both factors (p<0.01) were significant, while the type of EY factor and its interactions of age and washing were not. In goats, factors as the donor age (p<0.05) and sperm treatment (washed sperm+15% EY versus unwashed sperm+2%EY) (p<0.001) were significant, whereas there was no interaction between treatment and age.
