Resumen de Estudio comparativo de la morfometría de las subpoblaciones espermáticas en en la especie) bovina, caprina, ovina y porcina utilizando el método casma-f
S. Vicente Fiel, Inmaculada Palacín, P Santolaria, J.L. Yániz
This study was designed to compare the sperm nuclear morphometric subpopulations of four species of domestic artiodactyls (cattle, sheep, goat and pigs). Samples from 20 males of each species were collected. Smears were fixed with 2% glutaraldehyde, stained with Hoechst 33342 and photographed. At least 200 spermatozoa per sample were processed using the Image J analysis open software. Clustering procedures were performed to identify sperm subpopulations using the morphometric data obtained from each species. Our results show that, applying the CASMA-F technology and multivariate cluster analyses, it was possible to determine the subpopulations of spermatozoa with different morphometric characteristics in the four species studied. When comparing sperm subpopulations in these four species, the bull and the boar had two clearly differentiated size categories: large and small. However, in small ruminant species, 3 sperm nucleus size categories were established: large, average size and small. Male variability was identified in the distribution of sperm subpopulations described in the four species studied. It was concluded that the combination of CASMA-F technology with multivariate cluster analyses allow the study of morphometric sperm subpopulations and that there are important variations in the subpopulations among the four species studied.
