Resumen de Efecto de la adición de activina-a en el medio de maduración y del cultivo in vitro sobre el rendimiento de la pive en ovocitos de cabras prepúberes
S. Hammami Habibi, R. Morató, M.G. Catalá, María Teresa Paramio Nieto, D. Izquierdo
The aim was to evaluate the effect of activin-A added to the IVM and IVC media on cleavage rate, embryo development and blastocysts quality of oocytes from prepubertal goat. COCs were randomly allocated into four groups: (1) A0A0: COCs matured in IVM medium and cultured in SOF medium; (2) A0A10: COCs matured in IVM medium and cultured in SOF medium+10ng/mL activin-A; (3) A10A0: COCs matured in IVM medium+10ng/mL activin-A and cultured in SOF medium; and (4) A10A10: COCs matured in IVM medium + 10ng/mL activin-A and cultured in SOF medium+10ng/mL activin-A. Cleavage rate was recorded at 48hpi and blastocysts yield at Days 7, 8 and 9 pi. In order to assess the quality of blastocysts obtained from the treatment groups, cell number and the cellular composition of Day 8 pi blastocysts were evaluated by differential staining of ICM and TE. The cleavage rate did not show differences between treatments, however, blastocysts yield improved with the addition of activin-A. The highest blastocyst rate (18.7%) was obtained when activin-A was added only in IVC medium (A0A10). No differences in blastocyst mean cell number were detected among groups. In conclusion, the presence of activin-A during IVC improves developmental competence of prepubertal goat oocytes.
