Resumen de La biopsia embrionaria con láser permite el diagnóstico genético preimplantacional ovino sin reducir la supervivencia a término

M. J. Cocero, Purificación Sánchez Sánchez, José Folch Pera, B. Lahoz Gallo, Jorge Hugo Calvo Lacosta, F. Quintín, E. Sevilla Mur, José Luis Alabart Álvarez

• The effect of laser-assisted embryo biopsy on embryo survival at term was studied in ovine embryos with or without extruded cells at recovery. Two hundred viable compact morulas and blastocysts were recovered after superovulation of Rasa aragonesa ewes with pFSH. About half of embryos from each donor were biopsied (n=114; 66 with and 48 without extruded cells) while the rest were incubated at 38.5 ºC in TCM199 and 5% CO2/air (n=86; 60 with and 26 without extruded cells) during 18-22 h before transfer. Biopsy was performed in Dulbecco’s PBS, using an IR laser (1480 nm; 300 mW; 2.4 ms; XYClone, Hamilthon-Thorne). One to ten cells were recovered. After biopsy, embryos were incubated in the same conditions as non-biopsied embryos. The embryos that reached the expanded or hatched blastocyst stages after culture were transferred in pairs to recipient ewes. The embryo survival rate of the transferred embryos was similar in biopsied and intact embryos, irrespective of having (59.0%, 36/61 vs. 60.9%, 14/23; NS) or not (69.8%, 30/43 vs. 74.1% 43/58; NS) extruded cells. The effect of biopsy on the survival rate considering the total number of processed embryos was also non-significant. These results highlight the usefulness of laser in embryo biopsy.