Resumen de Efecto del fluido oviductal porcino pre- y post-ovulatorio sobre la resistencia enzimática de la zona pelúcida y la fecundación in vitro
L. Ballester, J.C. Martinez -Soto, C. Matas, R. Romar, P. Coy
Porcine oviductal fluid (OF) from animals at the late follicular phase of the estrous cycle or after ovulation was collected, and estrogen and progesterone levels were assessed. Pre-ovulatory OF contained higher concentrations of estradiol than progesterone, whereas the opposite occurred for post-ovulatory OF. Pre- and postovulatory OF was used to incubate porcine oocytes for 30 min. ZP digestion time (assessed by 0.5% pronase) and in vitro fertilization parameters assessed after incubation indicated that the pre-ovulatory OF, with higher estrogen concentration, increased the resistance of the ZP to enzymatic digestion and the levels of monospermy after IVF. These results suggest that, under physiological conditions, the effect of the oviductal fluid on the ZP enzymatic resistance can be a key factor for the success of fertilization by reducing the chances of polyspermy.
