Resumen de La selección de espermatozoides por las células oviductales modifica los parámetros de fecundación in vitro
The polyspermy is a major problem associated with in vitro fertilization in pigs. In vivo, the adhesion between sperm and OEC allows maintenance sperm viability, motility and fertile life span, because the oviduct modulates and control sperm capacitation until the time of ovulation. The spermatozoa bound to OEC appear to be an uncapacitation status. In vitro studies have shown that the cultured of oviduct epithelial cells have a beneficial effect on oocytes fertilization. The aim of this study was to evaluate whether the fertilizing capacity of sperm selected by oviduct cells differed from those not selected under in vitro conditions. Ejaculated sperm were incubated in oviductal cells, after which time sperm that were not bound to the oviductal cells (NU) and the sperm bound (U) were used for IVF. As IVF control were used sperm washed through Percoll ®. The results of in vitro fertilization show an increase in penetration and in number of spermatozoa bound to the zona pellucida, for sperm bound to oviductal cells (U). The highest value of monospermy was found in NU group, but also showed the lowest value of nuclear descondensation. In conclusion oviductal cells select the sperm subpopulation more apt to penetrate oocytes.
