María Luisa Tejido Mediavilla, Raúl Bodas Rodríguez, C. Blanco, P. Llorente, F. Javier Giráldez García
The in vitro gas production technique was used to test the effects of different doses (0, 2, 4 and 6%) of vegetable oils and soapstocks (sunflower oil –AG-, sunflower soapstock –OG-, olive oil –AO-, olive soapstock –OO-) added to the substrate for incubations (Total mixed rations, TMR: (26.3% wheat straw, 17.0% vetch hay and 56.7% concentrate) on ruminal fermentation and methane production. Ruminal fluid from cows fed on the TMR was used as inoculum. A mixture of lauric and miristic acids (4:1) at the same doses was used as positive control. Both vegetable oils and soapstocks linearly reduced gas (P<0.001) and methane production (P<0.05), raising acetate to propionate ratio (P<0.01). All tested supplements but AG decreased volatile fatty acid production (P<0.05). Although the differences between soapstocks and oils depend on the doses administered, generally speaking, the effects of AO were more pronounced than those of OO, and OG had more marked antimethanogenic effects than AG at 6% dose.
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