Expression pattern and clinical significance of beta 2-adrenergic receptor in oral squamous cell carcinoma: an emerging prognostic indicator and future therapeutic target
-
Akhilesh Krishna [2] ; Vineeta Singh [1] ; Nishtha Singh [1] ; Shraddha Singh [1] ; Sujit Kumar Mohanty [3] ; Rajender Singh [4] ; Vijay Kumar [1] ; Uma Shankar Singh [1] ; Rakesh Kumar Singh [1]
-
[1] King George's Medical University
King George's Medical University
India
-
[2] Speciality of Pathology and Cancer Genetics, Kalyan Singh Super Speciality Cancer Institute, Lucknow, UP, India
-
[3] Indiana Centre for Regenerative Medicine and Engineering, Department of Surgery, Indiana University School of Medicine, Indianapolis, IN, USA
-
[4] Division of Endocrinology, CSIR-Central Drug Research Institute, Lucknow, UP, India
-
- Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 24, Nº. 11 (November), 2022, págs. 2191-2199
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
-
Resumen
Purpose Beta 2-Adrenergic Receptor (β2-AR) is significantly overexpressed in various types of malignancies, which is associated with the worst prognosis. However, the role of β2-AR in oral cancer is not well identified. The present study aimed at investigating the β2-AR gene expression and its significance in relation with the clinicopathological features and overall survival of oral squamous cell carcinoma (OSCC) patients.
Methods Immunohistochemistry, western blot and quantitative real-time PCR techniques were used to analyze β2-AR protein and mRNA levels in a total of 65 histopathologically confirmed OSCC tissues (case group) and 65 normal tissues (control group) from the oral cavity.
Results Out of the total of 65 OSCC tissues, 41 tissues (63.1%) exhibited high expression for β2-AR protein. Percent positivity and relative density (mean ± SD) of protein were higher in the case group as compared to the control group (positivity 40.31 ± 3.01 vs. 20.46 ± 1.93, p < 0.001; density 2.77 ± 1.17 vs. 1.28 ± 0.37, p < 0.001). In addition, β2-AR mRNA level was also upregulated in patients compared to the controls (2.36 ± 1.30 vs. 1.09 ± 0.42, p < 0.001) and showed a positive correlation with immunostaining of protein in OSCC (r = 0.48, p = 0.011). High β2-AR protein expression was significantly associated with multiple risk habits (p = 0.045), histological differentiation (p = 0.013), clinical TNM stages (p = 0.014), and poor survival (p = 0.006) of patients. In the Cox proportional hazards model, β2-AR was identified as a prognostic biomarker of OSCC (p = 0.047).
Conclusion β2-AR protein level is identified as an independent significant prognostic factor in patients with oral carcinoma.
