Characteristics of gut microbiota in patients with gastric cancer by surgery, chemotherapy and lymph node metastasis

• Changchang Chen ^[1] ; Jian Shen ^[2] ; Yaoqiang Du ^[3] ; Xinwei Shi ^[4] ; Yaofang Niu ^[5] ; Gulei Jin ^[5] ; Yanxin Liu ^[6] ; Yongkang Shi ^[7] ; Jianxin Lyu ^[3] ; Lijun Lin ^[6]
  1. [1] School of Laboratory Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, China
  2. [2] Department of Medical Administration, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang, China
  3. [3] Laboratory Medicine Center, Department of Transfusion Medicine, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang, China
  4. [4] The Eye Hospital of Wenzhou Medical University (Zhejiang Eye Hospital), Hangzhou, Zhejiang, China
  5. [5] Hangzhou Guhe Information and Technology Company, Hangzhou, Zhejiang, China
  6. [6] School of Basic Medicine and Forensic Medicine, Hangzhou Medical College, Hangzhou, Zhejiang, China
  7. [7] General Surgery, Cancer Center, Department of Gastrointestinal and Pancreatic Surgery, Zhejiang Provincial People’s Hospital (Affiliated People’s Hospital, Hangzhou Medical College), Hangzhou, Zhejiang, China

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• Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 24, Nº. 11 (November), 2022, págs. 2181-2190
• Idioma: inglés
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• Resumen

  • Background Gastric cancer (GC) is a malignant gastrointestinal tumor that can result in high mortality. Surgery and chemotherapy are often used for the effective treatment of GC. In addition, lymph node metastasis is a significant factor affecting the therapy of GC. Current researches have revealed that gut microbiota has the potential as biomarkers to distinguish healthy people and GC patients. However, the relationship between surgery, chemotherapy, and lymph node metastasis is still unclear.

    Methods In this study, 16S rRNA sequencing was used to investigate 157 GC fecal samples to identify the role of surgery, chemotherapy, and lymph node metastasis. Immunohistochemical analysis was used to value the expression of Ki67, HER2 in GC patient tissues.

    Results There exist some gut microbiotas which can distinguish surgery from non-surgery GC patients, including Enterococcus, Megasphaera, Corynebacterium, Roseburia, and Lachnospira. Differences between lymph node metastasis and chemotherapy in GC patients are not significant. Moreover, we found the abundance of Blautia, Ruminococcus, Oscillospira were related to the expression of Ki67 and the abundance of Prevotella, Lachnospira, Eubacterium, Desulfovibiro were correlated with the expression of HER2.

    Conclusions The choice of treatment has a certain impact on the intestinal flora of patients with gastric cancer. Our research shows that surgery has a great effect on the intestinal flora of patients with gastric cancer. However, there were no significant differences in the characteristics of intestinal flora in patients with gastric cancer whether they received chemotherapy or whether they had lymph node metastasis. In addition, the association of gut microbiota with Ki67 and HER2 indicators is expected to provide the possibility of gut microbiota as a tumor prognostic marker.