Optimisation of regeneration and maintenance of morphogeniccallus in white poplar (Populus alba l.) by simple and double regeneration techiniques

• Pablo Fernández Rodríguez ^[1]
  1. [1] Tuscia University

     Tuscia University

     Viterbo, Italia

     
• Localización: XI young researchers meeting on conservation and sustainable use of forest systems / coord. por Elena Hidalgo Rodríguez, Francisco Javier Dorado Reyes, Ainhoa Iñiguez Soto, Diego A. Damián Carrión, Samuel Gato Martín, Guillermo Jové Alcalde, Raúl Arcadio Fernández González, 2017, ISBN 978-84-617-9574-1, pág. 41
• Idioma: inglés
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• Resumen

  • During my collaboration period in Viterbo, the main goal in the research was improving the maintenance in callus of two genotypes of white poplar from distinct geographical location in Italy:6K3 and 14P11, calculating the regeneration percentage in different conditions, altering the composition of the medium to observe which explant type was the best to preserve it as well. 6K3was located in North Italy in no saline conditions, finding it about 400 meters over the sea level, meanwhile 14P11 was situated in South Italy, close to the sea. These two genotypes were tested in light and dark conditions and four explant types were used: leaf, petiole, internode and internode longitudinally cut in the half. Besides, to ensure the variables that affect directly to regeneration, it was compared rooted and unrooted material. All this planning was check in fifteen different medium. Each of these had a growth regulator and a specific quantity. The main outline was cultured in MS (Murashige and Skoog) medium modified adding in the first trial 0,1 mg/L ofTDZ (Thidiazuron) and in the second one 1 mg/L of Z (Zeatin). The results said that medium with Z makes regeneration appears faster than in TDZ ones. Also, based on the percentage, there is more regeneration in Z than in TDZ medium. We could check that there are 96% of regeneration in 6K3 genotype, light conditions and internode cut in the half explants. Moreover, in both media were observed first the regenerated explants in the light than in the dark but it kept longer alive in the last one although they looked brownish. If we talk about explant types, internode and nternode cut in the half were the ones which have more regeneration percentage, in some case more than 90% ensured. However, leaf explants were really difficult to regenerate but it appeared unexpected roots from the leaves cultured. Comparing genotypes, it was observed that 6K3 genotype produces more regeneration than 14P11, particularly in the dark, they have produced more roots too but they turned yellow meanwhile the 14P11 samples kept the green colour.