Resumen de Influence of indolebutyric acid on the in vitro rooting of populus tremula l.
Plant rooting is one of the most critical phases of in vitro culture. The way to apply rooting hormones and their dose is key to success. We compared two forms of application of indolebutyric acid (IBA) to the in vitro rooting of Populus tremula L.: previous immersion of theexplant in IBA solution and IBA dissolved in the medium, with two different immersion times and two IBA concentration and immersion time to the rooting of Populus tremula L. Four clones explants were evaluated. For that, six media were used: rooting in media without hormones, rooting in media with 0.5 mg/l IBA concentration, immersion in a 0.5 mg/l solution for 5 and 15 seconds and immersion in a 0.1 mg/l solution for 5 and 15 seconds. All the aerial and submerged roots were measured, as well as the length of the largest root, stem length, number of leaves and presence of callus, contamination and necrosis in the in vitro phase for seven weeks and three times during ex vitro phase. From the results obtained, we concluded that the best treatment is the immersion of the explants in a solution with a concentration 1mg/l IBA for 15 seconds, followed rooting in a medium without hormones.
