Valladolid, España
Climate change can lead to an increase to vulnerability some species. Thus, it is necessary to study like both abiotic and biotic factors can cause forest decline. The main objective of this work was to develop a better understanding of the influence to biotic agents in the process of decline to Pinus pinaster Ait., especially the impact of fungal pathogens. Maritime pine is one of the most important forest species in the Iberian Peninsula taking into account both the protection it provides and the timber it produces. It is also the main conifer species in Spain in terms of area covered. In this study, the plots have 10 meters in Madrid (Spain), they were included three categories: decay trees whit less of 50% to foliage, healthy trees whit more of 50% to foliage and dead trees. In every plot, we were described health condition to forest stand and forest mensuration of trees individually. Also, we were measured foliage discoloration, size of the needles, fructification, damage agents and premature tree death. We have taken samples with symptoms of decay and without symptoms. Specifically, we were analyzed branches to detect defoliator agents, we were described the symptoms of cankers and we were sampled cores to root and neck region of trees. In the laboratory, we will isolate the fungi through growing medium and finally we will indentify for their morphological identification of identification with molecularanalysis.
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