Evaluation of antimicrobial activity against Enterococcus faecalis of activated chelating agents in different final rinse protocols: an ex vivo study

• Adriana Oliveira ^[1] ; Daniel Rocha ^[1] ; Alexandre De Martin ^[1] ; Ana-Grasiela Limoeiro ^[1] ; Wayne Nascimento ^[1] ; Carlos Fontana ^[1] ; Rina Pelegrine ^[1] ; Elizabeth Martinez ^[1] ; Carlos Bueno ^[1]
  1. [1] Department of Endodontics, Faculdade de Ilhéus Bahia, Brazil
• Localización: Journal of Clinical and Experimental Dentistry, ISSN-e 1989-5488, Vol. 14, Nº. 8 (August), 2022, págs. 646-651
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • To evaluate the antimicrobial activity of the following chelating agents against Enterococcus faecalis using quantitative polymerase chain reaction (qPCR) analysis: 1% peracetic acid (PA), 1% peracetic acid with 0.1% cetrimide (PAC), and 17% ethylenediaminetetraacetic acid (EDTA) activated by passive ultrasonic irrigation (PUI) or with Easy Clean (EC), all followed by 2.5% sodium hypochlorite (NaOCl).

    A total of 80 permanent human mandibular premolars were randomly divided into eight experimental groups according to the chemical solution and agitation protocol used: Group PA + PUI; Group PA + EC; Group PAC + PUI; PAC + EC; group EDTA + PUI; EDTA + EC, all followed by 2.5% NaOCl; and two control groups with saline solution(NaCl): NaCl + PUI and NaCl + EC. Microbial samples were collected before (S1) and after the irrigation protocol (S2). Intracanal E. faecalis reduction analysis was performed by qPCR. Intragroup analysis was performed using the Wilcoxon signed-rank test for paired data, and intergroup analysis was performed using the Wilcoxon Mann-Whitney U test for independent samples. The significance level was set at p< 0.05.

    A significant difference was found between S1 and S2 in all groups except NaCl+ EC (p = 0.1602). Comparison between groups showed that PAC + PUI was significantly different from PA +EC (p = 0.0448).

    The activated chelating agents were effective against E. faecalis, with significant results compared to the control groups. The peracetic acid with cetrimide activated by PUI showed better results than peracetic acid with EC.