Bone regeneration at extraction sockets filled with leukocyte-platelet-rich fibrin: an experimental pre-clinical study

• Gene Park ^[1] ; Ernesto B Benalcázar Jalkh ^[2] ; Daniel Boczar ^[3] ; Edmara TP Bergamo ^[2] ; Heoijin Kim ^[1] ; Gregory Kurgansky ^[1] ; Andrea Torroni ^[4] ; Luiz F Gil ^[5] ; Estevam A Bonfante ^[2] ; Paulo G Coelho ^[6] ; Lukasz Witek ^[1]
  1. [1] New York University

     New York University

     Estados Unidos

     
  2. [2] Universidade de São Paulo

     Universidade de São Paulo

     Brasil

     
  3. [3] MD. Hansjörg Wyss Department of Plastic Surgery, NYU Langone Medical Center, New York, NY USA
  4. [4] MD, PhD. Hansjörg Wyss Department of Plastic Surgery, NYU Langone Medical Center, New York, NY USA
  5. [5] DDS, MS, PhD. Department of Morphological Sciences, Federal University of Santa Catarina, Brazil
  6. [6] 0MD, DDS, PhD, MBA. University of Miami Miller School of Medicine, Miami, FL, USA; Department of Biomaterials, New York University College of Dentistry, New York, NY USA; Department of Mechanical and Aerospace Engineering, New York University Tandon School of Engineering, Brooklyn, NY; Hansjörg Wyss Department of Plastic Surgery, NYU Langone Medical Center, New York, NY USA

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• Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 27, Nº. 5 (September), 2022
• Idioma: inglés
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• Resumen

  • We aimed to histomorphometrically evaluate the effects of Leucocyte-Platelet-Rich Fibrin (L-PRF), with and without the combination of a bone grafting material, for alveolar ridge preservation using an in vivo canine model.

    Seven dogs (Female Beagles, ~18-month-old) were acquired for the study. L-PRF was prepared from each individual animal by drawing venous blood and spinning them through a centrifuge at 408 RCF-clot (IntrasSpin, Intra-Lock, Boca Raton, FL). L-PRF membranes were obtained from XPression fabrication kit (Biohorizons Implant Systems, Inc., AL, USA). A split mouth approach was adopted with the first molar mesial and distal socket defects treated in an interpolated fashion of the following study groups: 1) Empty socket (negative control); 2) OSS filled defect 3) L-PRF membrane; and 4) Mix of Bio-Oss® with L-PRF. After six weeks, samples were harvested, histologically processed, and evaluated for bone area fraction occupancy (BAFO), vertical/horizontal ridge dimensions (VRD and HRD, respectively), and area of coronal soft tissue infiltration.

    BAFO was statistically lower for the control group in comparison to all treatment groups. Defects treated with Bio-Oss® were not statistically different then defects treated solely with L-PRF. Collapsed across all groups, L-PRF exhibited higher degrees of BAFO than groups without L-PRF. Defects filled with Bio-Oss® and Bio-Oss® with L-PRF demonstrated greater maintenance of VRD relative to the control group. Collapsed across all groups, Bio-Oss® maintained the VRD and resulted in less area of coronal soft tissue infiltration compared to the empty defect. Soft tissue infiltration observed at the coronal area was not statistically different among defects filled with L-PRF, Bio-Oss®, and Bio-Oss® with L-PRF.

    Inclusion of L-PRF to particulate xenograft did not promote additional bone heading at 6 weeks in vivo. However, we noted that L-PRF alone promoted alveolar socket regeneration to levels comparable to particulate xenografts, suggesting its potential utilization for socket preservation.