Decompression induces inflammation but do not modify cell proliferation and apoptosis in odontogenic keratocyst

Daniela Trujillo González; Mariana Villarroel Dorrego; Raíza Toro; Gabriela Vigil; Vanesa Pereira Prado; Ronell Bologna Molina

Ayuda

Decompression induces inflammation but do not modify cell proliferation and apoptosis in odontogenic keratocyst

Daniela Trujillo-González ^[1] ; Mariana Villarroel-Dorrego ^[1] ; Raiza Toro ^[1] ; Gabriela Vigil ^[2] ; Vanesa Pereira-Prado ^[2] ; Ronell Bologna-Molina ^[2]
1. [1] Universidad Central de Venezuela
  
  Universidad Central de Venezuela
  
  Venezuela
2. [2] Área de Patología Molecular Estomatológica, Facultad de Odontología, UdelaR, Montevideo-Uruguay
Localización: Journal of Clinical and Experimental Dentistry, ISSN-e 1989-5488, Vol. 14, Nº. 1 (January), 2022, págs. 100-106
Idioma: inglés
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Resumen
- Background: Odontogenic keratocyst (OKC) is a development cyst, of odontogenic origin, that differs from other entities by its infiltrating and aggressive biological behavior. Among conservative treatments for large lesions, surgical decompression stands out, with a variable recurrence rate. Aim: To evaluate the histological effects of decompression treatment on OKC, including cell proliferation and apoptosis of epithelial cyst.
  
  Material and Methods: 21 OKC cases were included. Samples were taken before and after surgical decompression for histological evaluation and immunohistochemical staining of Ki-67, MCM4/7, Bax and Bcl2. Data were analyzed and compared using Student’s t and Wilcoxon tests for related samples, and p values <0,05 were considered statistically significant.
  
  Results: After decompression treatment an increase in inflammation of the cystic wall (p=0,029), loss of parake - ratinization of the epithelium ( p=0,007) and absence of palisade cell distribution in the basal layer were observed (p=0,002). There were no statistically significant changes in the expression of Ki-67 (p=0,323), MCM4/7 (p=0,079), Bax (p=0,392) or Bcl-2 when compared before and after decompression.
  
  Conclusions: Surgical decompression generates histological structural changes in OKC both in the epithelium and connective wall, however, these findings do not seem to alter induction of the cell cycle or epithelial apoptosis.