Efectividad antibacteriana entre sistema de irrigación ultrasónica pasiva y continua sobre Enterococcus faecalis. Estudio in vitro
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Ibarra de la Vega, Jessica Fernanda [1] ; Maldonado Paredes, José Eduardo [2] ; Nardello Leite, Laura Cristina [3] ; Romero Cazares, Roberto Xavier [1]
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[1] Universidad Central del Ecuador
Universidad Central del Ecuador
Quito, Ecuador
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[2] Universidad San Francisco de Quito
Universidad San Francisco de Quito
Quito, Ecuador
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[3] Universidad de São Paulo
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- Localización: Odontología, ISSN-e 1390-9967, ISSN 1390-7468, Vol. 23, Nº. 2, 2021
- Idioma: español
- Títulos paralelos:
- Antibacterial effectiveness between passive and continuous ultrasonic irrigation system on Enterococcus faecalis. In vitro study
- Enlaces
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Resumen
- español
Para tener éxito en la terapia endodontica es muy importante la desinfección del canal radicular. Objetivo. Comparar la efectividad antibacteriana entre Sistema de Irrigación Ultrasónica Pasiva e Irrigación Ultrasónica Continua sobre biofilm maduro de Enterococcus faecalis. Metodología. Se cultivó cepas de Enterococcus faecalis ATCC 29212 en canales radiculares de premolares uniradiculares durante 21 días. La preparación químico mecánica de los conductos radiculares fue realizada con el sistema Protaper Universal manual e irrigados con 2mL de hipoclorito de sodio al 2,5%. Las muestras fueron colectadas con conos de papel estériles, antes del preparo químico mecánico (S1), después del preparo químico mecánico (S2) y después del protocolo de irrigación final (S3). Los grupos fueron divididos posterior al preparo químico mecánico en dos grupos: G1 (n=10) Irrigación Ultrasónica Pasiva y G2 (n=10) Irrigación Ultrasónica Continua además de un grupo control positivo y un grupo control negativo. Las muestras obtenidas fueron sembradas en Agar Müller-Hinton e incubadas 24 horas a 37º C y se contaron las unidades formadoras de colonia (UFC). Los datos fueron analizados mediante la Prueba de Anova y T Student con un nivel de significancia <0,05%. Resultados. El biofilm maduro de Enterococcus faecalis fue removido en un 93,9 - 93,9% luego de la preparación químico mecánica, la activación ultrasónica pasiva consiguió reducir un 98,9% y con la activación ultrasónica continua se redujo a 99,2%. Las técnicas de desinfección complementaria utilizadas mostraron diferencias significativas comparadas con el preparo químico mecánico (p=0.000). La activación ultrasónica pasiva comparada con la activación ultrasónica continua no mostraron diferencias significativas (p=0,393). Conclusión. Los sistemas de activación complementaria resultan eficaces en la reducción de biofilm de Enterococcus faecalis.
- English
To be successful in endodontic therapy, root canal disinfection is very important. Objective. To compare the antibacterial effectiveness between the Passive Ultrasonic Irrigation System and the Continuous Ultrasonic Irrigation on a mature Enterococcus faecalis biofilm. Methodology. Experimental in vitro study. The sample consisted of 30 teeth, single-rooted premolars, which were standardized to a length of 16mm. The experimental units were subjected to an autoclaving process, and then strains of Enterococcus faecalis ATCC 29212 were cultivated by placing approximately 0.01 mL of standard value of the bacterial suspension in each root canal until obtaining mature biofilm for 21 days. The chemical-mechanical preparation was carried out by means of manual instrumentation with files from the universal Protaper system, following the manufacturer’s instructions until reaching the Protaper F3 file. During and after the use of each instrument, the canals were irrigated with 2mL of 2.5% NaOCl with Navitip 30-G needles. Root canal samples were collected with sterile #15 paper cones, before mechanical-chemical preparation (S1), after mechanical-chemical preparation (S2) and after the final irrigation protocol (S3). After (S2) the sample was divided into two groups: G1 (n=10) application of Passive Ultrasonic Irrigation, and G2 (n=10) application of Continuous Ultrasonic Irrigation, in addition to a positive control group and a negative control group. The samples obtained were seeded in Müller-Hinton Agar and incubated for 48 hours and subsequently the colony-forming units (CFU) were counted. The data obtained were processed in the statistical program SPSS. Analysis of results. The Shapiro-Wilk Normality test was carried out, in which the values of the level of significance (Sig) were higher than 0.05 (95% reliability); therefore, the samples come from populations with Normal distribution, Parametric tests were used to compare groups: ANOVA, Student’s T. Differences between the processes were obtained as a result: Chemical-mechanical preparation eliminated an average of 93.9% of mature Enterococcus faecalis biofilm, while passive ultrasonic activation eliminated 98.9% and continuous ultrasonic activation eliminated 99.2%. Therefore, the complementary disinfection techniques used showed significant differences compared to the chemical-mechanical preparation (p=0.000). Passive Ultrasonic Activation does not show significant differences (p = 0.393) compared to Continuous Ultrasonic Activation. With these results, it is concluded that the two complementary activation systems turn out to have the same antibacterial efficacy on Enterococcus faecalis.
- español
