Scopoletin inhibits PDGF-BB-induced proliferation and migration of airway smooth muscle cells by regulating NF-κB signaling pathway

• Zhongxiang Fan ^[2] ; Dan Tang ^[1] ; Qiang Wu ^[2] ; Qun Huang ^[3] ; Jie Song ^[2] ; Qiping Long ^[2]
  1. [1] Chengdu University of Traditional Chinese Medicine

     Chengdu University of Traditional Chinese Medicine

     China

     
  2. [2] Department of Pediatrics, Chengdu Wenjiang Maternal and Child Health Hospital, Chengdu, Sichuan Province, China.
  3. [3] Chengdu Wenjiang Maternal and Child Health Hospital, Chengdu, Sichuan Province, China

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• Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 50, Nº. 1, 2022, págs. 92-98
• Idioma: inglés
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• Resumen

  • Background: Asthma is a common chronic inflammatory disease of the airway, and airway remodeling and the proliferation mechanism of airway smooth muscle cells (ASMCs) is of great significance to combat this disease.

    Objective: To assess possible effects of scopoletin on asthma and the potential signaling pathway.

    Materials and methods: ASMCs were treated PDGF-BB and scopoletin and subjected to cell viability detection by CCK-8 assay. Cell migration of ASMCs was determined by a wound closure assay and transwell assay. The protein level of MMP2, MMP9, calponin and α-SMA were measured using western blot. The levels of NF-κB signaling pathway were detected by Western blotting.

    Results: Scopoletin inhibited proliferation of PDGF-BB - induced ASMCs. Also it suppressed the migration and invasion of PDGF-BB - induced ASMCs. We further showed that Scopoletin regulated phenotypic transition of ASMCs. Mechanically, Scopoletin inhibited proliferation and invasion of ASMCs by regulating NF-κB signaling pathway.

    Conclusions: We therefore thought Scopoletin could serve as a promising drug for the treatment of asthma.