Resumen de Effect of cardiac stem cell derived extracellular vesicles on cardiomyocytes
M. Moro López, L. Gómez Cid, A. S. de la Nava, María Eugenia Fernández Santos, Lilian Grigorian Shamagian, F. Fernández Avilés
Cell and extracellular vesicle-based therapies have shown antiarrhythmic potential but have not been extensively studied. Hence, in this work it is investigated the influence of extracellular vesicles secreted by atrial cardiosphere-derived cells (CDC-EVs) on cardiomyocytes. The HL-1 cell line was used, adding CDCEVs after 48h of culture. When completely confluent, cell cultures were analyzed with optical mapping and posteriorly with MATLAB software for the measurement of the main electrophysiological properties: dominant frequency, complexity, curvature, rotor meandering and conduction velocity (CV). Then, a two-step RT-qPCR was performed to quantify the expression of genes involved in the cardiac impulse propagation (SCN5A, CACNA1C and GJA1, coding for the sodium and calcium channels and connexin 43, respectively). As significant results, CV and meandering increased and complexity and curvature decreased after the CDC-EVs administration, supported by an increase in the expression of the three genes under study. This suggests that the CDC-EVs treatment induces a less arrhythmogenic profile driven by changes in the gene expression, increasing CV and modifying rotor dynamics so complexity is decreased. In spite of having an in vitro nature and working with non-human cells, this study opens new insights into the antiarrhythmic potential of CDC-EVs.
