Optical coherence tomography imaging of colorectal neoplastic polyps developed in genetically modified rats
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[1] Tyndall National Institute, Cork, Ireland
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[2] Jesús Usón Minimally Invasive Surgery Centre, Cáceres, Spain
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- Localización: XXXVIII Congreso Anual de la Sociedad Española de Ingeniería Biomédica. CASEIB 2020: Libro de actas / Roberto Hornero Sánchez (ed. lit.), Jesús Poza Crespo (ed. lit.), Carlos Gómez Peña (ed. lit.), María García Gadañón (ed. lit.), 2020, ISBN 978-84-09-25491-0, págs. 53-56
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
The high incidence and mortality of colorectal cancer requires the need to develop new technologies for the early detection and diagnosis of disease in early stages. In this way, cure rates of patients could be increased. Despite the imaging techniques used today for the detection and diagnosis of colorectal cancer, there is still an unmet need for a technique that could provide images of tissue below the visible surface with a higher resolution, and with the possibility of distinguish adenomatous polyps and allow rapid diagnosis in situ previous to the histopathological examination. One of these emerging techniques with great potential is Optical Coherence Tomography (OCT). In this work, a developed neoplastic polyp in the colon of a genetically modified PIRC F344/NTac-Apcam rat has been imaged using OCT in order to observe its subsurface structure. The polyp was imaged with a Thorlabs commercial OCT system at 1300 nm obtaining an axial and lateral resolution of 3 m and 4 m respectively. Results show that the neoplastic polyp demonstrated irregular glands with enlarged nuclei in the epithelial layer showing different morphology that affect the underlying homogenous layer structure of healthy colon tissue. 3D rendering of the sample provided by the OCT system allows obtaining an “optical biopsy” for better analysis of the surface and subsurface structure of the colonic polyp. The inclusion of a scanning OCT probe through an endoscopic working channel could be used to perform in-vivo imaging of polyps during colonoscopy as a support for an improved diagnosis.
