The genotypes and virulence attributes of C. albicans isolates from oral leukoplakia

• Manjula Manoji Weerasekera ^[3] ; Gayan Kanchana Wijesinghe ^[3] ; Asanga Sampath ^[3] ; Ayomi Dilhari ^[4] ; Thilina Madhumal ^[1] ; Rasika Dilrukshi ^[3] ; Rajitha Willaddara ^[3] ; Sunil Karunathilaka ^[5] ; Chinthika Gunasekara ^[3] ; Neluka Fernando ^[3] ; Lakshman Samaranayake ^[2]
  1. [1] University of Colombo

     University of Colombo

     Sri Lanka

     
  2. [2] University of Hong Kong

     University of Hong Kong

     RAE de Hong Kong (China)

     
  3. [3] Department of Microbiology, Faculty of Medical Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
  4. [4] Department of Basic Sciences, Faculty of Allied Health Sciences, University of Sri Jayewardenepura, Gangodawila, Nugegoda, Sri Lanka
  5. [5] Oral and Maxillofacial Unit, Colombo South Teaching Hospital, Kalubowila, Dehiwala, Sri Lanka

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• Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 26, Nº. 6 (November), 2021
• Idioma: inglés
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• Resumen

  • There is a debate as to whether some types of oral leucoplakias (OL) are caused by Candida species, and whether they contribute to the malignant transformation, associated with a minority of such lesions. As no detailed population analysis of yeast isolates from OL is available, we evaluated the virulence attributes, and genotypes of 35 C. albicans from OL, and compared their genotypes with 18 oral isolates from healthy individuals.

    The virulence traits evaluated were esterase, phospholipase, proteinase, haemolysin and coagulase production, and phenotypic switching activity, and yeast adherence and biofilm formation. DNA from OL and control yeasts were evaluated for A, B or C genotype status.

    Phospholipase, proteinase, and coagulase activity and biofilm formation was observed in 80%, 66%, 97 % and 77 % of the isolates, respectively. Phenotypic switching was detected in 8.6%, while heamolytic, and esterase activity and adherence were noted in all isolates.

    The genotype A was predominant amongst both the OL and control groups. Due to the small sample size of our study a larger investigation to define the role of candidal virulent attributes in the pathogenicity of OL is warranted, and the current data should serve as a basis until then.