Morphological analysis and immunohistochemical expression in restorations with self-adhesive resin: a randomized split-mouth design-controlled study

• Natália-Gomes de Oliveira ^[2] ; Marina-Torreão da Silveira ^[3] ; Leorik-Pereira da Silva ^[1] ; Keylla-Marinho-Albuquerque Barros ^[4] ; Laís-Maciel Costa ^[2] ; Marília-de Lima Soares ^[4] ; Marianne-de Vasconcelos Carvalho ^[5]
  1. [1] Universidade Federal de Campina Grande

     Universidade Federal de Campina Grande

     Brasil

     
  2. [2] MSc, PhD student, Dental School, Universidade de Pernambuco/FOP-UPE; Camaragibe, PE, Brazil
  3. [3] MSc, Dental School, Universidade de Pernambuco/FOP-UPE; Camaragibe, PE, Brazil
  4. [4] MSc, PhD, Dental School, Universidade de Pernambuco/FOP-UPE; Camaragibe, PE, Brazil
  5. [5] MSc, PhD, Adjunct Professor, Dental School, Universidade de Pernambuco/FOP-UPE; Camaragibe, PE, Brazil

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• Localización: Journal of Clinical and Experimental Dentistry, ISSN-e 1989-5488, Vol. 13, Nº. 9 (September), 2021, págs. 927-934
• Idioma: inglés
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• Resumen

  • To evaluate the morphology and immunohistochemical expression of MMP-9, TIMP-3, COL-I, TNF-alfa and COX-2 in the dentinopulp complex of restorations with self adhesive composite (Vertise Flow/Kerr) compared with conventional resin composite with self-etching adhesive (Filtek Z250/3M ESPE and Clearfil SE Bond/Kuraray-Noritake).

    A randomized, controlled, double-blind, split-mouth, two-arm clinical trial was conducted. Twenty-seven volunteers with third molars indicated for extraction received two deep class I restorations, one with each material. The morphology and immunohistochemical expression were measured at 15 days after the restorative procesures. The data were submitted to the Fisher`s Exct test (α=0.05).

    Through morphological analysis, a slight disorganization of the odontoblastic layer was visualized, along with a slight inflammatory infiltrate in the VERT and CSEB groups (p< 0.05). In immunohistochemical analysis, TNF-alpha, MMP-9, TIMP-3 and COX-2 demonstrated more cases of positive pulp labeling.

    Self-adhesive resin composite and conventional resin composite with a self-etching bonding agent promoted slight alterations in the dentin-pulp complex, indicating a repair ability and reversibility of the inflammatory process against aggressions of the adhesive restorative procedure.