Cromatografia de exclusion molecular de (bio)polielectrolitos

• Rosa García, Iolanda Porcar ^[1] ; Agustín Campos ^[1] ; Juan E. Figueruelo ^[1] ; Iolanda Porcar Boix
  1. [1] Universitat de València

     Universitat de València

     Valencia, España

     
• Localización: Revista Iberoamericana de Polímeros, ISSN 1988-4206, ISSN-e 0121-6651, Vol. 3, Nº. 1, 2002, págs. 48-58
• Idioma: español
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• Resumen

  • The elution behaviour of several standards ionic polymers, such as poly (L-glutamic acid) (PGA), sodium poly(styrene sulfonate) (PSSNa) and poly(acrylic acid) (PAA) has been analyzed by aqueous size exclusion chromatography (ASEC) as a function of pH and ionic strength (I) of eluent, on both inorganic- and organic-based packings. Quantitative evaluation of secondary effects, such as ionic contributions, is undertaken taking into account electric double-layer effects and the effective radius of polyion. A second order dependence of the elution volume on I-1/2is obtained in fair agreement with experimental results. Suggestions on quantitative evaluation of hydrophobic effects are also pointed out.