Tyrophagus putrescentiae group 4 allergen allergenicity and epitope prediction

• F.-X. Teng ^[3] ; H.-F. Huang ^[1] ; D.-Z. Ge ^[2] ; L.-L. Yu ^[3] ; C. Xu ^[2] ; Y.-B. Cui ^[1]
  1. [1] Nanjing Medical University

     Nanjing Medical University

     China

     
  2. [2] University of Iowa

     University of Iowa

     City of Iowa City, Estados Unidos

     
  3. [3] Jiangsu Vocational College of Medicine, China

  Mostrar afiliaciones +
• Localización: Allergologia et immunopathologia: International journal for clinical and investigate allergology and clinical immunology, ISSN-e 1578-1267, ISSN 0301-0546, Vol. 48, Nº. 6, 2020, págs. 619-625
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • Introduction and objectives Allergen-specific immunotherapy (ASIT) is the only allergic disease-modifying therapy available for children and adults, and recombinant allergens are an interesting approach to improve allergy diagnosis and ASIT. Tyrophagus putrescentiae is a common storage mite that produces potent allergens. The aim of this study was to express and characterize recombinant group 4 allergen protein of T. putrescentiae (Tyr p 4), and to further investigate allergenicity and potential epitopes of Tyr p 4.

    Materials and methods The cDNA encoding Tyr p 4 was generated by RT-PCR and subcloned into pET-28a(+) plasmid. The plasmid was then transformed into E. coli cells for expression. After purification by nickel affinity chromatography and identification by SDS-PAGE, recombinant Tyr p 4 protein was used for a skin prick test and an ELISA to determine the allergic response.

    Results Study participants’ allergic response rate to Tyr p 4 protein was 13.3% (16/120). Eight B-cell epitopes and three T-cell epitopes of Tyr p 4 were predicted.

    Conclusions Similar to group 4 allergens of other species of mite, allergenicity of Tyr p 4 is weak. The expression, characterization and epitope prediction of recombinant Tyr p 4 protein provide a foundation for further study of this allergen in the diagnosis and ASIT of storage mite allergy.