How Should Apples Be Prepared for a Fruit Salad? A Guided Inquiry Physical Chemistry Experiment

• Renée S. Cole ^[1] ; Marc Muniz ^[2] ; Erica Harvey ^[3] ; Robert Sweeney ^[4] ; Sally Hunnicutt ^[5]
  1. [1] University of Iowa

     University of Iowa

     City of Iowa City, Estados Unidos

     
  2. [2] Rutgers University

     Rutgers University

     City of New Brunswick, Estados Unidos

     
  3. [3] Fairmont State University

     Fairmont State University

     Estados Unidos

     
  4. [4] University of Southern California

     University of Southern California

     Estados Unidos

     
  5. [5] Virginia Commonwealth University

     Virginia Commonwealth University

     Estados Unidos

     

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• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 97, Nº 12, 2020, págs. 4475-4481
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • In this guided inquiry experiment, students extract catecholase enzyme from apples to catalyze the oxidation of catechol. They follow the reaction using the UV–vis absorbance of the p-benzophenone produced to determine the Michaelis–Menten kinetic parameters. Students make selected experimental choices within a structured framework such as selecting the apple varietal, the pH of the reaction mixture, and the reaction inhibitor. The experiment has been tested at multiple universities in physical chemistry laboratory courses with both large and small enrollments. We describe the experiment and its implementation in both synchronously and asynchronously taught courses.