Thin layer chromatography (TLC) is a technique widely used in industrial and research chemistry. It is an inexpensive and simple technique, which makes it attractive for undergraduate chemistry lab experiments. Unfortunately, the technique is sometimes incorrectly taught. Incorrect techniques impede the use of TLC in research laboratories. The most common error in teaching TLC technique is solvent vapors inside the chamber not being equilibrated prior to use. The second most common mistake is the use of watch glass covers when beakers are used for TLC developing chambers. Simply replacing the watch glass with aluminum foil, and preparing the developing chamber before the TLC plates, allows time for the developing solvent to saturate the chamber which yields faster, more reproducible results.
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