Cadaverine reverse transporter (CadB protein) contributes to the virulence of Aeromonas veronii TH0426

• LuoTao Tao ^[1] ; YuanHuan Kang ^[1] ; Lei Zhang ^[1] ; Qiumei Shi ^[2] ; Ying Li ^[1] ; Tonglei Wu ^[2] ; Aidong Qian ^[1] ; WuWen Sun ^[1] ; Xiaofeng Shan ^[1]
  1. [1] Jilin Agricultural University

     Jilin Agricultural University

     China

     
  2. [2] Hebei Normal University of Science & Technology
• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 23, Nº. 4, 2020, págs. 489-499
• Idioma: inglés
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• Resumen

  • Aeromonas veronii is one of the main pathogens causing sepsis and ulcer syndrome in freshwater fish. Analysis of the results of epidemiological investigations in recent years has revealed that the virulence of A. veronii and its tolerance to drugs have been increasing year by year. Currently, most of the research on A. veronii focuses on its isolation, identification, and drug susceptibility, whereas research on its virulence factors and pathogenesis mechanisms is relatively rare. In this study, we identified and obtained the highly expressed TH0426 cadaverine reverse transporter (CadB) of A. veronii. We used efficient suicide plasmid-mediated homologous recombination to delete the cadB gene in TH0426 and constructed a cadB deletion strain. The LD50 of ΔcadB was 93.2 times higher than that of TH0426 in zebrafish, the toxicity of ΔcadB was 9.5 times less than that of TH0426 in EPC cells, and the biofilm formation ability of ΔcadB was 5.6-fold greater than that of TH0426. In addition, motility detection results indicated that ΔcadB had lost its swimming ability. The results of flagellar staining and TEM demonstrated that ΔcadB shed the flagella. In summary, the virulence and adhesion of A. veronii TH0426 were significantly decreased by the deletion of cadB, which might provide a theoretical basis for research into A. veronii virulence factors.