Resumen de Study of short- and long-term storage of teeth and its influence on DNA
Leticia Olga Rubio Lamia, Luis Javier Martínez González, Esther Martínez Espín, Stella Martín de las Heras
DNA degradation can interfere with the resolution of forensic cases. Allelic dropout often reduces the opportunity for adequatecomparisons between degraded and reference samples. This study analyzed DNA degradation in 24 extracted teeth after storage at room temperaturefor 0, 2, 5, and 10 years. DNA concentration, quantified by dot-blot hybridization, declined significantly for the first 2 years, but there was no signifi-cant further degradation from the second to the tenth year of storage. COfiler analysis was used and the allelic dropout ratio for the amelogeninlocus relative to CSF1PO locus was also estimated. Statistically significant differences were found between fresh teeth and teeth from the 2- and5-year groups but not from the 10-year group. Under our storage conditions most of the DNA degradation occurred during the first 2 years. Furtherresearch is needed to control for individual and external factors that could affect DNA
