Analysis of bacterial communities of infected primary teeth in a Mexican population

• Enid Karina Salas-López ^[1] ; Sergio Casas-Flores ^[2] ; Nguyen Esmeralda López-Lozano ^[3] ; Esther Layseca-Espinosa ^[1] ; Christian A. García-Sepúlveda ^[1] ; Perla del Carmen Niño-Moreno ^[1] ; Amaury Pozos-Guillén ^[1]
  1. [1] Universidad Autónoma de San Luis Potosí

     Universidad Autónoma de San Luis Potosí

     México

     
  2. [2] PhD, IPICYT, División de Biología Molecular, San Luis Potosí, México
  3. [3] PhD, IPICYT, División de Ciencias Ambientales, San Luis Potosí, México

  Mostrar afiliaciones +
• Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 25, Nº. 5 (September), 2020
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • The objective of this study was to describe the bacterial communities associated with pediatric patients with endodontic infections of temporal teeth by targeting the 16S rRNA gene using pyrosequencing.

    Microbiological samples were obtained from the lower primary molars of thirteen 13 pediatric patients with dental infections. An aspiration method for microbiological sampling was used. The identification of microbiota employing the pyrosequencing method by targeting the 16S gene was performed.

    Ribosomal 16S RNA gene sequences were amplified, obtaining a total of 16,182 sequences from 13 primary infected molars (13 different individuals) by pyrosequencing. Bacteroidetes phyla (35.15%) were the most abundant followed by Firmicutes (33.3%) and Fusobacteria (10.05%); the presence of specific pathogenic bacteria was determined as well.

    The infected root canal of primary teeth contains a high diversity of anaerobic bacteria, and Bacteroidetes, Firmicutes, and Fusobacteria phyla were the most abundant; Prevotella and Streptococcus genera were the most prevalent.