MIB1 proliferation index in breast infiltrating carcinoma: Comparison with other proliferative markers and association with new biological prognostic factors

• González-Vela, M.C. ^[1] ; Garijo, M.F. ^[1] ; Fernández, F. ^[1] ; Val-Bernal, José Fernando ^[1]
  1. [1] Universidad de Cantabria

     Universidad de Cantabria

     Santander, España

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 16, Nº. 2, 2001, págs. 399-406
• Idioma: inglés
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  • Aims: In breast invasive carcinoma our objectives were I) to compare cellular proliferation determined by MIBI index with S-phase fraction (SPF) assessed by flow cytometry and with mitotic index, and II) to examine the association of MIBI index with classical and with new biological prognostic factors [bcl2, p53, c-erbB-2 and cathepsin D (CD)]. Methods and results: From 102 cases of breast invasive carcinoma, 5,um thick serial sections were cut from formalin-fixed, paraffin-embedded tissue blocks, and processed for detection of CD, c-erbB-2, p53, bcl-2, Ki-67 antigen MIB-l and estrogen receptors (ER) and progesterone receptors (PR). SPF was measured by flow cytometry in fresh-frozen tissue samples taken from the carcinoma in each patient. MIBI index was correlated with SPF (rho=0.45, p