Modified Method for Extraction of Photosynthetic Plant Pigments for Microcolumn Chromatography
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James A. Ciaccio [1] ; Kareem Hassan [1]
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[1] Fordham University
Fordham University
Estados Unidos
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- Localización: Journal of chemical education, ISSN 0021-9584, Vol. 97, Nº 8, 2020, págs. 2362-2365
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
Column chromatography of photosynthetic plant pigments is a common experiment in introductory laboratory courses for science majors and nonscience majors alike. We report a simple, rapid, reproducible, and small-scale solid–liquid extraction of photosynthetic pigments from inexpensive, store-bought dried herbs that affords a dark-green extract solution, all of which can be applied directly to a pipet microcolumn made from as little as 0.75 g of silica gel. Carotenes and chlorophylls are cleanly separated using standard steps in microcolumn chromatography: the former elute together within 20 min using gravity and 9:1 (v/v) heptane:EtOAc, and the latter elute together within seconds using air pressure and EtOAc.
