Melanization stimulating factors in the integument of the Mugil cephalus and Dicertranchus labrax
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[1] Universidad de Murcia
Universidad de Murcia
Murcia, España
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- Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 15, Nº. 4, 2000, págs. 1145-1150
- Idioma: inglés
- Enlaces
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Resumen
The pigment pattern expression resides in the chromatoblasts of the embryonic skin. The differentiation of these chromatoblasts is influenced by specific local factors such a melanization inhibiting factor (MIF) and a melanization-stimulating factor (MSF). We reveal the presence of these factors by means of a series of experiments on the skin of the marine species of fish Dieertranchus labrax and MugU cephalus, each with different pigment pattern, the former having a light skin and the latter a darker one. Media conditioned by exposure to dorsal and/or ventral skin, stimulates the melanization of Xenopus laevis neural crest cells throughout a 3 day assay period. Similarly conditioned culture media tested on B16-FlO murine malignant melanocytes, revealed a considerable influence in enzymatic activities: dopachrome tautomerase (OCT), tyrosine hydroxylase and dopa oxidase. The use of media in a dose response basis suggests that the conditioned media may contain both melanophore stimulating and inhibiting factors. The results obtained may actually reflect the resultant activity of the two factors present.
