Foreign serum-induced bile duct lesion (BDL) in athymic BALB/c nude mice

• K. Honjo ^[2] ; K. Doi ^[1]
  1. [1] University of Tokyo

     University of Tokyo

     Japón

     
  2. [2] University of Alabama, South Street, Birmingham, Alabama, USA
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 15, Nº. 2, 2000, págs. 463-467
• Idioma: inglés
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• Resumen

  • To investigate a role of cellular immunity in foreign serum-induced bile duct lesion (BDL) in mice, athymic BALB/c nude (nu/nu) mice were intraperitoneally injected with swine serum (SS) twice a week up to 8 weeks and were compared with euthymic BALB/c heterozygote (nu/+) and wild-type (+/+) mice treated with S S in the same way for 4 weeks. All immunized nu/+ and +1+ mice developed marked BDL, and their sera showed high anti-SS IgE and IgG I antibody titers, whereas no immunized nu/nu mice developed lesions, and their sera showed no elevation of antibody titers. Next, nu/nu mice were reconstituted with splenocytes derived from nu/+ mice, and then were intraperitoneally injected with SS twice a week for 3 weeks. Most of the reconstituted nu/nu mice developed BDL, and their sera showed the elevation of anti-SS IgE and IgG antibody titers. These results suggest that cellular immunity may playa pivotal role in the pathogenesis of swine serum-induced BDL.