Immunohistochemical study on the distribution of galanin-containing nerves in the chicken pancreas
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Hiramatsu, K. [1] ; Ohshima, K. [1]
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[1] Shinshu University
Shinshu University
Japón
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- Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 10, Nº. 2, 1995, págs. 283-288
- Idioma: inglés
- Enlaces
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Resumen
The distribution of galanin-containing nervous elements in the chicken pancreas was investigated by use of immunohistochemical method. Galanin-immunoreactive nerve fibres formed a perivascular plexus and a dense network in the subepithelial layer of secretory ducts. The muscle layers of secretory ducts were also supplied with galaninimmunoreactive nerve fibres. The exocrine pancreas received a supply of varicose nerve fibres showing galanin immunoreactivity. In the endocrine part, B-islets were innervated by galanin-immunoreactive nerve fibres, whereas A-islets received fewer nervous elements. Double staining combined with the immunofluorescence method for galanin and acetylcholinesterase histochemistry showed at least two types of ganglion cells in the interlobular connective tissue; one showing both acetylcholinesterase activity and galanin immunoreactivity, and the other showing acetylcholinesterase activity only. The present results demonstrate that the chicken pancreas is innervated by galanin-containing nerves of intrinsic origin and suggest that galanin coexists with acetylcholine in the chicken pancreas.
