Apoptosis in gallbladder carcinomas and dysplasias, its relation to the expression of caspases 3, 6 and 8 and apoptosis regulating proteins bcl-2, mcl-1 and bax

• N. Turunen ^[1] ; P. Paakko ^[1] ; Y. Solni ^[1]
  1. [1] University cf Oulu and Oulu University Hospital,Oulu, Finland
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 15, Nº. 1, 2000, págs. 53-60
• Idioma: inglés
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• Resumen

  • In this study we investigated apoptosis and the expression of caspases 3, 6 and 8 and bcl-2, mcl-l and bax in 39 gallbladder carcinomas and 7 epithelial dysplasias. The average apoptotic index was 0.68±0.91 %. The extent of apoptosis was higher in grade II-III than grade I tumours or epithelial dysplasias (p=0.003). Also, tumours invading beyond serosa or into other organs (TI-T4) had a higher apoptotic index than other tumours (p=O.05). Caspase 3 expression was found in 37 (95%) and caspase 6 and 8 expression each in 30 (77%) carcinomas. Their expression associated with each other and tended to increase along with the progression of the lesions. Bc1-2 expression was found in only 4 (10%) tumours. In contrast, mcl-l positivity was found in 34 (87%) and bax positivity in all cases.

    The results show that apoptosis is increased along with progression of the neoplastic lesion of the gallbladder epithelium. Caspases 3,6 and 8 are strongly expressed in gallbladder carcinomas suggesting that they contribute to the increased apoptosis observed in them. Of the bcl-2 family proteins, bcl-2 was expressed infrequently suggesting that it does not play any significant role in apoptosis inhibition in gallbladder tumours.