Ultrastructural and immunocytochemical study of the leptomeres in the mouse cardiac muscle fibre

• Hosokawa, T. ^[1] ; Okada, T. ^[1] ; Kobayahsi, T. ^[1] ; Hashimoto, K. ^[1] ; Seguchi, H. ^[1]
  1. [1] Kochi Medical School Hospital

     Kochi Medical School Hospital

     Japón

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 9, Nº. 1, 1994, págs. 85-94
• Idioma: inglés
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• Resumen

  • The three-diiiiensional configuration and cheiriicol composition of leptoniei-es in the inouse \eiitric~ilar cardiac inuscle fibre were electron iiiicroscopically und iminuiiocytochemically in\-estigated ~ising seinithin sections and specific antibodies agairist actin. the intei-mediate filament proteins desinin and viiiientin. anci the aciin-binding proteins a-actiriin, filainin and vinculin. The leptomeres appeared columnar in shape arid periodically segmeiited by electron-dense tiisc-like septa. The electrori-lucent areas betweeri these septa uere composed of fiiie interlinked filainents running obliquely to the major axis cif the Ieptomere.

    Actiii was localized in the electron-dense lii-ies of the leptoineres but not in the fine filaments. No reaction was. however. detected for desinin. vinientin. \zi~iculin.

    rilainin and a-actinin. The preserit results suggest.

    therefore. that the leptomeres inay not have a contractile function.