Vitexin, an inhibitor of hypoxia‑inducible factor‑1α, enhances the radiotherapy sensitization of hyperbaric oxygen on glioma

T. Xie; J.R. Wang; C.G. Dai; X.A. Fu; J. Dong; H.-Q. Huang

Ayuda

Vitexin, an inhibitor of hypoxia‑inducible factor‑1α, enhances the radiotherapy sensitization of hyperbaric oxygen on glioma

T. Xie ^[2] ; J.R. Wang ^[2] ; C.G. Dai ^[1] ; X.A. Fu ^[2] ; J. Dong ^[1] ; Q. Huang ^[1]
1. [1] Second Affiliated Hospital of Soochow University
  
  Second Affiliated Hospital of Soochow University
  
  China
2. [2] Department of Neurosurgery, Suzhou Municipal Hospital, The Affiliated Suzhou Hospital of Nanjing Medical University, 242 Guangji Road, Suzhou 215008, Jiangsu, China
Localización: Clinical & translational oncology, ISSN 1699-048X, Vol. 22, Nº. 7, 2020, págs. 1086-1093
Idioma: inglés
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Resumen
- PurposeVitexin, an inhibitor of hypoxia-inducible factor (HIF)-1α, has anti-tumor effect. However, whether it can enhance the radiotherapy sensitization of hyperbaric oxygen (HBO) on glioma is unclear. This study aimed to investigate the effect of vitexin.MethodsThe nude mice with paw-transplanted glioma were divided into four groups: control group, HBO + radiation group, HBO + vitexin group, and HBO + vitexin + radiation group. The mice of last two groups were daily given vitexin 75 mg/kg by intraperitoneal injection. 30 min after administration of vitexin, the HBO-treated mice were daily placed in HBO chamber for 60 min. The radiation-treated mice were given local tumor irradiation once every week during the HBO treatment, and the dose of irradiation was 10 Gy/time. The experimental treatment lasted for 21 days.ResultsCompared with the HBO + radiation group, the tumor volume, tumor weight, and tumor weight coefficient in the HBO + vitexin + radiation group were lower (p < 0.05). Importantly, the contents of reduced glutathione and glutathione peroxidase as well as expressions of HIF-1α, vascular endothelial growth factor, glucose transporter (GLUT)-1, and GLUT-3 proteins in tumor tissues were also lower in the HBO + vitexin + radiation group than in the HBO + radiation group (p < 0.01).ConclusionsVitexin can cooperate with HBO to sensitize the glioma radiotherapy, and its mechanisms may be correlated to the inhibition of HIF-1α protein expression and subsequent decrements of its downstream protein expressions, which finally cause the reduction of antioxidant capacity.