Reverse lectin histochemistry: Design and application of glycoligands for detection of cell and tissue lectins

• Gabius, H.J. ^[4] ; Gabius, S. ^[4] ; Zemlyanukhina, T.V. ^[1] ; Bovin, N.V. ^[1] ; Brinck, U. ^[5] ; Danguy, A. ^[6] ; Joshi, S.S. ^[2] ; Kaiser, K. ^[7] ; Schottelius, J. ^[8] ; Sinowatz, F. ^[9] ; Tietze, L.F. ^[10] ; Vidal-Vanaclocha, F. ^[3] ; Zanetta, J.P. ^[11]
  1. [1] Institute of Bioorganic Chemistry

     Institute of Bioorganic Chemistry

     Rusia

     
  2. [2] University of Nebraska Medical Center

     University of Nebraska Medical Center

     City of Omaha, Estados Unidos

     
  3. [3] Universidad del País Vasco/Euskal Herriko Unibertsitatea

     Universidad del País Vasco/Euskal Herriko Unibertsitatea

     Leioa, España

     
  4. [4] Institut für Pharmazeutische Chemie, Abteilung Glykobiochemie und Angewandte Tumorlektinologie, Philipps-Universitat, Marburg, German
  5. [5] Zentrum Pathologie der Universitat, Goettingen, German
  6. [6] Laboratoire de Biologie Animale et Histologie Comparée, Faculté des Sciences de I'Université Libre de Bruxelles, Bruxelles, Belgium
  7. [7] Abteilung Pathologie, Thoraxklinik, Heidelberg, German
  8. [8] Abteilung Protozoologie, Tropeninstitut, Hamburg, Germany
  9. [9] lnstitut für Tieranatomie der Universitat, München, German
  10. [10] lnstitut für Organische Chemie der Universitat, Goettingen, Germany
  11. [11] Laboratoire de Neurobiologie Moléculaire des Interactions Cellulaires, Centre de Neurochimie du CNRS, Strasbourg Cedex, France

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• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 8, Nº. 2, 1993, págs. 369-383
• Idioma: inglés
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  • Plant and invertebrate lectins are valuable cyto- and histological tools for the localization of defined carbohydrate determinants. The welldocumented ubiquitous occurrence of sugar receptors encourages functional considerations. Undoubtedly, analysis of the presence of vertebrate lectins in tissues and cells is required to answer the pertinent and tempting question on the physiological relevance of protein (1ectin)-carbohydrate recognition in situ. Carrierimmobilized glycoligands, derived from custom-made chemical synthesis, enable the visualization of respective binding sites. Histochemically inert proteins or synthetic polymers with appropriate functional groups are suitable carrier molecules for essential incorporation of ligand and label. The resulting neoglycoconjugates can track down tissue receptors that are neither impaired by fixation procedures nor blocked by endogenous highaffinity ligands. Lectins, especially the receptors of the tissue under investigation (endogenous lectins), and appropriately tailored immobilized glycoligands or lectin-specific antibodies (when available) are complementary tools to test the attractive hypothesis that diverse, functionally relevant glycobiological processes within or between cells are operative. Concomitant evaluation of both sides of lectin histochemistry, namelylectins as tools and lectins as functionally important molecules in situ, will indubitably render desired progress amenable in our often still fragmentary understanding of the importance of tissue lectin and glycoconjugate expression and its regulation.