Immunoreactivity of Thomsem-Friedenreich (TF) antigen in human neoplasms: The importance of carrier-specific glycotope expression on MUC1

• Baldus, S.E. ^[1] ; Hanisch, F.G. ^[1] ; Monaca, E. ^[1] ; Karsten, U.R. ^[2] ; Zirbes, T.K. ^[1] ; Thiele, J. ^[1] ; Dienes, H.P. ^[1]
  1. [1] University of Cologne

     University of Cologne

     Kreisfreie Stadt Köln, Alemania

     
  2. [2] Max Delbrück Center for Molecular Medicine

     Max Delbrück Center for Molecular Medicine

     Berlin, Stadt, Alemania

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 14, Nº. 4, 1999, págs. 1153-1158
• Idioma: inglés
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• Resumen

  • On the basis of their known fine specificities we evaluated the immunohistochemical marker qualities of two monoclonal antibodies (mabs) defining the tumor-associated TF disaccharide Galf31-3GaINAc. This antigen is expressed in certain tumors in correlation with prognosis and metastasis. The reactivity of one of these mabs (A78-G/ A7) depends on clustered TF disaccharides (glycosylation at vicinal Ser/Thr positions) while the other - mab BW835 - has been characterized to bind specifically to TF disaccharide linked to a motif within the MUCI repeat. Therefore, mab BW835 represents an interesting tool for the identification of tumor-associated glycoforms of MUCl, which are involved in tumor progression and metastasis, but also in the recognition of tumor cells by cytotoxic T cells.

    As references the TF-binding lectins from peanut (PNA) and Artocarpus integrifolia (jacalin) were applied. The binding patterns of these immunoreagents were strikingly distinct. Mab BW835 showed a significantly stronger reactivity than mab A 78-G/A 7, especially in gastric, mammary, pancreatic, thyreoideal, renal and bladder carcinomas. PNA and jacalin receptors exhibited an expression in the majority of all cancer types, with the exception of seminoma and glioblastoma/sarcoma. These results can be explained by the broader fine specificities of the lectins. Furthermore, a strong expression of MUCl-bound TF antigen is indicated by the staining pattern of mab BW835. The marker qualities of both antigens, TF and MUC1 , are combined in the binding specificity of BW835, and hence this antibody may have a high impact for the immunodetection of these tumor-associated antigens.