Resumen de Immunocytochemical distribution of cytochrome P4501A (CYP1A) in developing gilthead seabream, Sparus aurata
María Carmen Sarasquete Reiriz, José Antonio Muñoz Cueto, Juan B. Ortiz Delgado, F.J. Rodríguez Gómez, M.T. Dinis, H. Segner
CYPlA is a major inducible enzyme in the metabo lism of xenobiotic substrates. In this pape r we investigat e by mean s of immunohistochemistry, the tissue distribution of constitutive cytochrome P4501A (CYP lA) during the period of endogenous nutrition (from hatching until day 4) in developing gilthead seabrcam , Sparus aurata larvae . For this purpose, a polyclonal antiserum (BN-1, Biosense Laboratories) directed against conse rved piscine CYP IA sequences was used on paraffin-embedded sections from seabream larva e. From hatching onward, CYP I A immunoreactivity was observed in the following tissues and cells: syncytial, oil-globule envelopes and matrix of the yolk-sac, kidney (epithelia of renal tubules), cardiac muscle cells, skin epidermal cells, troncal musculature, enterocytes of different intestinal regions, goblet cells of the bucco-pharyngeal region, gill epithelial cells and the endothelia of the vascular system of various tissues (especially from liver and brain). Moreover, eye (retina), olfactory epithe lium and some positive nerve fibers locat ed in the proximity of the olfactory bulbs and running ventrally toward th e post erior brain were strongly CYPIA immunoreactive. In general, the intensit y of immunostaining incre ased with larval development.
