Do adhesion molecules importantly regulate leukocyte kinetics within intraacinar microvessels of the lung?

• Yamaguchi, K. ^[1] ; Nishio, K. ^[1] ; Aoki, T. ^[1] ; Suzuki, Y. ^[1] ; Sato, N. ^[1] ; Takeshita, K. ^[1] ; Kudo, H. ^[1]
  1. [1] Keio University

     Keio University

     Japón

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 13, Nº. 4, 1998, págs. 1089-1102
• Idioma: inglés
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• Resumen

  • Precise assessment of blood cell kinetics in the pulmona ry mi c rocircul ation is ex treme ly difficult because pulmonary microvascular architecture contains arte rioles, ve nul es a nd c apill ari es in a n exceeding ly intricate and densely convoluted fas hion. Conventional e piluminescence mi c roscopy may not be suit abl e fo r investiga tion o f bl ood ce ll kineti cs in the pulmonary mi c roc irc ul a ti o n, in w hi c h a rt e ri o les, ve nul es a nd capillary networks are not located in the same plane. To overcome these impediments, we recently developed a real-time confocal laser luminescence microscope with a high-speed analysis component having the capac ity to y ie ld co nfoca l-images o f ra pidl y movin g ce lls a t a ra te o f 1,000 frames/sec a nd a t s uffi c ie ntl y hi g h magnifica ti o n. In th e c urr e nt rev iew, we will first introduce the details of our newly developed obse rvation system constructed with a view to estimation of blood cell dynamics in the intraacinar microcirculation of the lung. Applying this novel method to isolated perfused rat lungs, we will secondly address the issue of whether or not leukocyte-endothelium inte ractions in the pulmonary microcircul ation qualitative ly differ from those serv ing in the systemi c mi crocircul ation. We will particul a rl y s hed light o n possib le roles of e ndoth eli al ICAM-l , e ndotheli a l P-se lec tin a nd le ukocy te L-se lec tin in distorting leukocy te kineti cs in the intraacinar microvesse ls under a variety of diseased conditions, including pr o lo nge d ex pos ur e to a h y pe rox ic e n v iro nm e nt inducing a significant upregulation of ICAM- I as we ll as P-selectin on the pulmonary microvascular endothelium, and stimulation of leukocytes by an IL-8 analog causing downreg ul a tion of le ukocy te L-se lectin but inve rse upregul ation of C018-related integrins.