Localization of thrombospondin-1 and its cysteine-serine-valine-threonine-cysteine-glycine receptor in colonic anastomotic healing tissue

• Roth, J.J. ^[1] ; Buckmire, M.A. ^[1] ; Rolandelli, R.H. ^[2] ; Granick, M.S. ^[2] ; Tuszynski, G.P. ^[1]
  1. [1] Allegheny University of the Health Sciences, Medical College of Pennsylvania and Hanhnemann School of Medicine, Department of Surgery, Division of General Surgery, Division of Plastic Surgery, Philadelphia, USA
  2. [2] Division of Plastic Surgery, Philadelphia, USA
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 13, Nº. 4, 1998, págs. 967-971
• Idioma: inglés
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• Resumen

  • Thrombospondin-I (TSP-I) is a matrix protein implicated in mechanisms of wound healing.

    TSP-I contains the sequence cysteine-serine-valinethreonine-cysteine-glycine (CSVTCG) that has been shown to function primarily as a cell adhesion domain.

    Our laboratory has isolated a novel receptor specific for the CSVTCG adhesive domain of TSP-l. Immunohistochemical staining techniques and computerized image analysis were used to identify and quantitate TSP-l and its CSVTCG receptor in surgically created colon anastomotic wounds. Histopathologic and quantitative examination demonstrated increased expression of TSP1 and its CSVTCG receptor in areas of wound healing.

    These findings suggest a rol e for TSP-l and its CSVTCG receptor in wound healing. The control of expression and activity of these molecules may eventually be the basis for the development of wound healing agents that could significantly reduce the morbidity from surgical intervention.