Sialoglycoconjugate dimorphism of the mouse submandibular gland acinar cells. Ultrastructural evidence by lectin-protein A-gold probes and sialidase digestion

• Menghi, G. ^[1] ; Bondi, A. M. ^[1] ; Marchetti, L. ^[1] ; Gabrielli, M. G. ^[1] ; Materazzi, G. ^[1]
  1. [1] Università di Camerino

     Università di Camerino

     Camerino, Italia

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 13, Nº. 1, 1998, págs. 137-146
• Idioma: inglés
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• Resumen

  • An ultrastructural analysis of lectin receptors on th e submandibular glands fro m mice of both sexes was performed utilizing horseradish pero xidase-labell ed lectins in conjunction with antiperox idase antibody and protein A-gold . Both qu alitati ve and quantit ati ve sexrelated differe nces in terminal suga r expression within secre tory gran ul es were detected. Following sia lidase digestion. also suhtenninal acceptor suga rs for terminal siali c ac ids, proved to be differentiall y ex pressed in the submandibular g land s of mal es and fema le s.

    Heteroge neo us distributi on of sialoglycoconjugates characteri zed bv the terminal disaccharide sialic acid-/3- galactose was f~ llIndt o occur in female ac inar ce lls. Also DBA reacti ve sites indicating the presence of terminal CI.-N-ace tylgalactosamin e discriminated betwee n male and female ac in ar sec re tor y glycoco nju ga tes. This difference was emphasized by sialidase pretreatment that ev idenced a marked occurrence of sialic acid subtended to u- N-ace tvlga lac tosamine in males in contrast to a modest pr es-e ~ce in fe males. The different sial ylation pa tt e rn s of acinar cell sec reto ry prod uc ts, pro bab ly related to a different exp ressio n of 0- and N-linked sia logl ycoconjugates, g iv e in sig ht into th e sex ual dimorphism of the mouse submandibular gland known until rece ntl y for the convoluted granular tubules.