The microanatomy of calcium stores in human neutrophils: Relationship of structure to function

• Pettit, E. J. ^[1] ; Davies, E. V. ^[1] ; Hallett, M. B. ^[1]
  1. [1] University of Wales

     University of Wales

     Castle, Reino Unido

     
• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 12, Nº. 2, 1997, págs. 479-490
• Idioma: inglés
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• Resumen

  • As changes in cytosolic free Ca2+ play key roles in coupling responses in neutrophils, it is important to locate and identify Ca2+ storage sites within these cells. Here, recent data is presented which highlights the functional link between microanatomical structure and cell signalling function. Fluorescent optical probes for cytosolic free Ca2+ have been used, together with organelle specific markers. We present evidence from conventional fluorescence microscopy, together with ratiometric and confocal laser scanning fluorescence microscopy, which pin-points two cellular locations for Ca2+ within the neutrophil; one within the nuclear lobes, and the other towards the cell periphery. Knowledge of these two locations provides a clear insight into how signalling in this cell type is regulated and provides a framework for explaining how specific stimuli act to produce specific responses.