Diverse Bacteria Associated with Root Nodules of Spontaneous Legumes in Tunisia and First Report for "nifH-like" Gene within the Genera "Microbacterium" and "Starkeya"
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Frédéric Zakhia [1] ; Habib Jeder [2] ; Anne Willems [3] ; Monique Gillis [3] ; Bernard Dreyfus [1] ; Philippe de Lajudie [1]
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[1] Laboratoire des Symbioses Tropicales et Méditerranéennes
Laboratoire des Symbioses Tropicales et Méditerranéennes
París, Francia
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[2] Institut des Régions Arides
Institut des Régions Arides
Túnez
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[3] Ghent University
Ghent University
Arrondissement Gent, Bélgica
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- Localización: Microbial ecology, ISSN-e 1432-184X, ISSN 0095-3628, Vol. 51, Nº. 3, 2006, págs. 375-393
- Idioma: inglés
- Texto completo no disponible (Saber más ...)
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Resumen
We characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of Tunisia by 16S rDNA polymerase chain reaction (PCR)–restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), 16S rDNA and 16S–23S rDNA internal transcribed spacer sequencing. Phylogenetically, these isolates belong to the branches containing the genera Inquilinus, Bosea, Rhodopseudomonas, Paracraurococcus, Phyllobacterium, Ochrobactrum, Starkeya, Sphingomonas, Pseudomonas, Agromyces, Microbacterium, Ornithinicoccus, Bacillus, and Paenibacillus. These strains did not induce any nodule formation when inoculated on the wide host spectrum legume species M. atropurpureum (Siratro) and no nodA gene could be amplified by PCR. However, nifH sequences, most similar to those of Sinorhizobium meliloti, were detected within strains related to the genera Microbacterium, Agromyces, Starkeya and Phyllobacterium.
