Resumen de Concurrent exercise training and Murf-l and Atrogin-1 gene expression in the vastus lateralis muscle of male Wistar rats
Yashar Moradi, Farzad Zehsaz, Mir Alireza Nourazar
Introduction The present study was to determine the effect of 8-week of the concurrent exercise training on Murf-l and Atrogin-1 Gene Expression of the vastus lateralis muscle in male Wistar rats.
Material and methods This study was conducted as an experimental project consisting of four groups of 35 two-month-old male Wistar rats. The animals were randomly divided in 4 groups: (1) endurance training, (2) resistance training, (3) combined training, and (4) control. The animals in the training groups took part in training programs for 8-week. 48h after the last exercise session, the Murf-1 and Atrogin-1 genes of the vastus lateralis muscle were examined through the use of qPCR method.
Results The results obtained from this study revealed that after 8-week of endurance exercise, Murf-1 and Arogin-1 gene expression significantly increased compared to the control group (p=0.04 and p=0.043). In contrast, in the resistance training group, the gene expression of Murf-1 and Atrogin-1 decreased significantly in comparison with the control group (p=0.02 and p=0.04). In addition, the concurrent training group showed no difference in Murf-1 and Atrogin-1 gene expression after 8-week of exercise compared to that of the control group (p=0.43 and p=0.38).
Conclusions Based on the results of the present research, it can be expressed that resistance training prevents muscular atrophy by decreasing Murf-1 and Atrogin-1 gene expression. Conversely, endurance exercises cause an increase in the expression of these genes, thereby leading to atrophy in the muscles. The results also showed that concurrent exercises do not have a meaningful effect on muscular atrophy.
