Enzymatic Saccharification of Dilute Acid Pretreated Saline Microalgae, Nannochloropsis Oculata

• Samriddhi Buxy ^[1] ; Robert Diltz ^[2] ; Pratap Pullammanappallil ^[1]
  1. [1] University of Florida

     University of Florida

     Estados Unidos

     
  2. [2] United States Air Force Academy

     United States Air Force Academy

     Estados Unidos

     
• Localización: Global Partnerships for Development and Engineering Education: Proceedings of the 15th LACCEI International Multi-Conference for Engineering, Education and Technology,July 19-21, 2017, Boca Raton, FL, United States / Victor Hugo Gonzalez Jaramillo (dir.), Pedro Fabricio Zanzzi Diaz (dir.), Rudy Altamirano Zambrano (dir.), Gissela Ganchozo Ortega (aut.), 2017, ISBN 978-0-9993443-0-9
• Idioma: inglés
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• Resumen

  • Present study evaluated saccharification potential of marine microalgae N. oculata by employing conventional techniques of hydrolysis and subsequent saccharification often used for lignocellulosic biomass. N.oculata was first hydrolyzed using dilute acid such as, 5% (v/v) sulfuric acid, and 5% (v/v) and 2% (v/v) phosphoric acid at 160C before subjecting to enzymatic saccharification by two commercial cellulases, EI and EII. Neither dilute acid hydrolysis nor enzymatic saccharification alone released any sugars. However, hydrolysates after acid hydrolysis were readily saccharified on addition of enzymes EI or EII. The extent of saccharification ranged between 8 and 100% in all experiments. Sulfuric acid hydrolysis produced furfurals whereas no side products were detected after phosphoric acid hydrolysis. Maximum sugar yield using EI was 345 g sugars/kg ash free dry matter (afdm) within 4 hours whereas EII yielded 360 g sugars/kg afdm within 12 hours. Twice of the nominal enzyme loading facilitated 35% more sugar release and half the nominal enzyme loading yielded 64% less sugars. It was concluded that conventional dilute phosphoric acid hydrolysis followed by enzymatic saccharification using commercially available enzymes could be efficient for saccharification of marine microalgae.