Caesalpinia coriaria fruits and leaves extracts possess in vitro ovicidal activity against Haemonchus contortus and Haemonchus placei

Rolando Rojo-Rubio ^[1] ; José F. Vázquez-Armijo ^[1] ; Manases González-Cortazar ^[2] ; Alejandro Zamilpa ^[2] ; Agustín Olmedo-Juárez ^[3] ; Pedro Mendoza-de Gives ^[3] ; Javier Arece-García ^[4] ; Germán David Mendoza-Martínez ^[5] ; Héctor Aaron Lee ^[6]
1. [1] Universidad Autónoma del Estado de México
  
  Universidad Autónoma del Estado de México
  
  México
2. [2] Instituto Mexicano del Seguro Social
  
  Instituto Mexicano del Seguro Social
  
  México
3. [3] Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
  
  Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias
  
  México
4. [4] Universidad de Matanzas
  
  Universidad de Matanzas
  
  Cuba
5. [5] Universidad Autónoma Metropolitana
  
  Universidad Autónoma Metropolitana
  
  México
6. [6] Universidad Autónoma de San Luis Potosí
  
  Universidad Autónoma de San Luis Potosí
  
  México
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Localización: Veterinaria México, ISSN 0301-5092, Vol. 6, Nº. 4, 2019, págs. 18-29
Idioma: varios idiomas
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Resumen
- The ovicidal effect of hydro-alcoholic extracts from mature fruits and dried leaves of the Caesalpinia coriaria tree, against Haemonchus contortus and H. placei was evaluated by the egg hatching inhibition test (EHIT). Secondary compounds within the extracts were identified by high performance liquid chromatography. Five extract concentrations were assessed in the inhibition tests (25.0, 12.5, 6.2, 3.1 and 1.5 mg/mL), using distilled water and Thiabendazole (0.5 mg/mL) as negative and positive controls, respectively. The results were analysed under a 2 × 2 × 6 completely randomized factorial design, and mean differences were established by the Tukey’s post hoc test. The 50% (EC50) and 90% (EC90) effective concentrations for each extract were determined using a Probit analysis. A concentration-dependent ovicidal effect of hydro-alcoholic extracts from both leaves and fruits was observed, reaching a 100% efficacy against both nematode species with the 25.0 mg/mL concentration (p < 0.05). The EC50 for the fruit and leaves extracts were established as 1.63 and 3.91 mg/mL, and as 3.98 and 11.68 mg/mL, against H. contortus and H. placei, respectively. The most important secondary compounds identified in extracts were free-condensed tannins, gallic acid and methyl-gallate. In conclusion, hydro-alcoholic extracts from C. coriaria mature fruits and dried leaves inhibit in vitro egg hatching of H. contortus and H. placei.